Development of a Rapid and Simultaneous Detection Method for Buprenorphine, Norbuprenorphine and Naloxone in Human Plasma Using Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometer with Solid-phase Extraction

作者：Guo Qiaozhen; Du Zhenxia^*

来源：Chinese Journal of Chemistry, 2011, 29(9): 1922-1926.

• ultra-high performance liquid chromatography-tandem mass spectrometer
• buprenorphine
• naloxone
• norbuprenorphine
• human plasma

A simultaneous method was successfully established and validated for the separation and determination of buprenorphine (BP), its primary metabolite, nor-buprenorphine (NBP) and a proposed co-formulate, naloxone (NLX) in human plasma. The method used buprenorphine-d(4) (BP-D(4)), nor-buprenorphine-d(3) (NBP-D(3)), naltrexone (NTX) as internal standards (ISs). 100 mu L of plasma sample fortified with the ISs was cleaned up by solid-phase extraction (SPE), and was then separated on a Waters Acquity (TM) BEH C(18) column with gradient elution using methanol and water (containing 0.2% formic) at a flow rate of 0.25 mL center dot mn(-1). The mass spectrometer was used for detection and was operated in the positive electrospray ionization with multiple reaction monitoring (MRM) mode. The three compounds were effectively separated in 5 min. The linear ranges of the compounds were 0.1-25, 0.25-25 and 0.05-25 ng center dot mL(-1) for BP, NBP and NLX, respectively, with r >= 0.9935. The method had high sensitivity (the limits of detection were 0.02, 0.1 and 0.01 ng center dot mL(-1) for BP, NBP and NLX, respectively) and high recoveries (>= 97.6%). The result was shown to be linear and satisfactorily met current acceptance criteria for validation of bio-analytical method: intra and inter assay precisions within the required limits of <= 25% RSD. The LOQs fulfilled the LOQ requirements: precision <= 25% RSD, and was fully validated according to the State Food and Drug Administration (SFDA) regulations. The results demonstrated that ultra-high performance liquid chromatography-tandem mass spectrometer (UPLC-MS/MS) with SPE was a powerful detection tool and contributed to pharmaceutical analysis in biological matrices.

• 出版日期2011-9
• 单位北京化工大学