A novel preparation method for microspheres by glycerol modified solid-in-oil-in-water multi-emulsion

作者：Yuan, Weien^*; Ren, Tiantian; Wu, Fei; Zhao, Hao; Jin, Tuo

来源：Polymers for Advanced Technologies, 2010, 21(5): 371-376.

DOI：10.1002/pat.1438

• protein delivery
• polysaccharide microparticles
• PLGA microspheres
• S/O/W

Biodegradable material poly(D, L-lactic-co-glycolic) acid (PLGA) plays an important role in drug-sustained release systems. Here, we describe a glycerol modified solid-in-oil-in-water (m-S/O/W) emulsion method for PLGA microspheres, in order to encapsulate proteins in PLGA by utilizing dextran glassy particles to protect the proteins from denaturing, unfolding, and aggregation during preparation and new external water phase to prevent the inner dextran glassy particles from leaking into the external water phase. External water phase containing 20, 40, 60, 80% glycerol showed that proteins released faster and more completely with increased glycerol content. According to their varied release profiles, microspheres of different formulations could be used to encapsulate vaccines or for delivering proteins over long-term.

• 出版日期2010-5
• 单位上海交通大学