Mitochondrial DNA D-loop and 12S Regions Analysis of the Long-Crowing Local Breed Denizli Fowl from Turkey

作者：Karaman Mesut^*; Kirdag Nurside

来源：Kafkas Universitesi Veteriner Fakultesi Dergisi, 2012, 18(2): 191-196.

• 12S rDNA
• D-loop
• Denizli fowl
• Local breed
• mt-DNA

Aim of this study is to analyze the genetic structure of long-crowing Denizli chicken, a Turkish local fowl, using nucleotide sequences of the mitochondrial DNA 12S and D-loop regions. DNA isolation was carried out using feather samples of cocks of this local breed. D-loop and 12S regions were amplified using polymerase chain reaction technique and ca 1230 bp and 950 pb PCR products were obtained respectively. Native genotype Denizli fowl's D-loop and 12S regions were sequenced and sequencing data were analyzed and compared with the related published data. Nucleotide content of the 12S region showed a 32% A, 19%T, 19% G and 30%C, whilst AT and GC ratios were found as 59.88% and 40.12% respectively for D-loop region. D-loop sequence data analysis clearly identified the Denizli fowl within the clade of long-crowing cocks. The results give the first informative data about the mitochondrial DNA D-loop and 12S regions of this local breed from Turkey.

• 出版日期2012-4