科研之友
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摘要
Young-of-the-year (age-0) striped bass, Morone saxatilis, were studied to characterize their responses to inflammatory stimuli. There were two studies, with the hypotheses that (i) <5g striped bass would respond to inflammatory insult within 6h, and (ii) response to antigens would be maintained for >24h in larger fish. Study I was conducted to understand the cytokine expression pattern (IL-1, TNF-, Nramp and TGF-) in response to lipopolysaccharide (LPS) or Freund's complete adjuvant (FCA) stimulation in age-0 striped bass (3.44 +/- 1.68g, 70.6 +/- 10.3mm fork length) up to 24h post injection (hpi). Study II was similar to Study I, but striped bass were sampled over a longer time frame (by 48hpi) and larger age-0 striped bass were used (20.6 +/- 5.9g, 129.2 +/- 10.9mm fork length). It was confirmed that immunostimulants such as LPS and FCA induce production of inflammatory cytokines and Nramp, which are important in innate immune response to bacterial infection. The responses were rapidly stimulated with LPS (IL-1, TNF-, TGF- >3-fold increase, compared to PBS) or FCA (IL-1 >3-fold and TGF- >2-fold, compared to PBS) within 6hpi and maintained in most cases 48hpi (spleen Nramp and TGF- 2-fold >PBS, at 24 and 48h), similar to other teleosts. Intraperitoneal injection with PBS also simulated inflammatory gene expression, but was delayed (IL-1, TNF-, TGF- and Nramp, FCA and LPS< at 6h; 24h >LPS and PBS) in comparison to LPS and FCA, suggesting that this procedure and possibly the volume used can be stimulatory and potentially harmful in age-0 fish. Therefore, this study suggests that age-0 striped bass are capable of strong cytokine induction in response to immunological stimulation within a very short period of time.
- 出版日期2015-1
