Black fly salivary gland extract inhibits proliferation and induces apoptosis in murine splenocytes

作者：Tsujimoto H; Gray E W; Champagne D E^*

来源：Parasite Immunology, 2010, 32(4): 275-284.

DOI：10.1111/j.1365-3024.2009.01186.x

• apoptosis
• black fly
• saliva
• T lymphocyte

P>Black flies are known to be vectors of pathogens including Onchocerca volvulus, which causes human onchocerciasis, and Vesicular Stomatitis Virus. Their salivary secretion has been shown to contain a complex cocktail of anti-haemostatic factors and immunomodulatory activities, which may contribute to efficient transmission of the pathogens. Black fly salivary gland extract (SGE) inhibits mitogen-stimulated mouse splenocyte proliferation, including proliferation of both CD4+ and CD8+ T cells. The factor responsible for the inhibition was determined to be a protein (or protein complex) of a size larger than 50 kDa. Moreover, exposure to SGE results in activation of caspase 3 and characteristic morphological changes in CD4+ and CD8+ T cells, suggesting that induction of apoptosis could, at least in part, be responsible for this inhibition.

• 出版日期2010-4