A Three-State Model with Loop Entropy for the Overstretching Transition of DNA

作者：Einert Thomas R^*; Staple Douglas B; Kreuzer Hans Juergen; Netz Roland R

来源：Biophysical Journal, 2010, 99(2): 578-587.

DOI：10.1016/j.bpj.2010.04.046

We introduce a three-state model for a single DNA chain under tension that distinguishes among B-DNA, S-DNA, and M (molten or denatured) segments and at the same time correctly accounts for the entropy of molten loops, characterized by the exponent c in the asymptotic expression S similar to -c In n for the entropy of a loop of length n. Force extension curves are derived exactly by employing a generalized Poland-Scheraga approach and then compared to experimental data. Simultaneous fitting to force-extension data at room temperature and to the denaturation phase transition at zero force is possible and allows us to establish a global phase diagram in the force-temperature plane. Under a stretching force, the effects of the stacking energy (entering as a domain-wall energy between paired and unpaired bases) and the loop entropy are separated. Therefore, we can estimate the loop exponent c independently from the precise value of the stacking energy. The fitted value for c is small, suggesting that nicks dominate the experimental force extension traces of natural DNA.

• 出版日期2010-7-21