A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

作者：Endres Bradley; Basseres Eugenie; Rashid Tasnuva; Chang Long; Alam M Jahangir; Garey Kevin W^*

来源：Jove-Journal of Visualized Experiments, 2017, (123): e55383.

DOI：10.3791/55383

• Issue 123
• Clostridium difficile
• scanning electron microscopy
• antibiotics
• vancomycin
• metronidazole
• ridinilazole
• fidaxomicin

Assessment of antibiotic action with new drug development directed towards anaerobic bacteria is difficult and technically demanding. To gain insight into possible MOA, morphologic changes associated with antibiotic exposure can be visualized using scanning electron microscopy (SEM). Integrating SEM imaging with traditional kill curves may improve our insight into drug action and advance the drug development process. To test this premise, kill curves and SEM studies were conducted using drugs with known but different MOA ( vancomycin and metronidazole). C. difficile cells (R20291) were grown with or without the presence of antibiotic for up to 48 h. Throughout the 48 h interval, cells were collected at multiple time points to determine antibiotic efficacy and for imaging on the SEM. Consistent with previous reports, vancomycin and metronidazole had significant bactericidal activity following 24 h of treatment as measured by colony-forming unit (CFU) counting. Using SEM imaging we determined that metronidazole had significant effects on cell length (> 50% reduction in cell length for each antibiotic; P<0.05) compared to controls and vancomycin. While the phenotypic response to drug treatment has not been documented previously in this manner, they are consistent with the drug's MOA demonstrating the versatility and reliability of the imaging and measurements and the application of this technique for other experimental compounds.

• 出版日期2017-5