Chondroprotective Activity of Murraya exotica through Inhibiting beta-Catenin Signaling Pathway

作者：Wu, Longhuo^*; Liu, Haiqing; Zhang, Rui; Li, Linfu; Li, Jialin; Hu, Haibo; Huang, Hao

来源：Evidence-Based Complementary and Alternative Medicine, 2013, 2013: 752150.

DOI：10.1155/2013/752150

• WNT/BETA-CATENIN
• ARTICULAR CHONDROCYTES
• OSTEOARTHRITIS
• ACTIVATION
• TRANSCRIPTION
• DEGRADATION
• SUPPRESSION
• QUERCETIN
• MEDIATORS
• MECHANISM

Osteoarthritis (OA) is a degenerative joint disease that affects millions of people. Currently, there is no effective drug treatment for it. The purpose of this study is to investigate the chondroprotective effects of Murraya exotica (L.) on OA. The rat OA models were duplicated to prepare for separating OA chondrocytes, synovial fluid (SF), and serum containing M. exotica (50 mg/kg, 100 mg/kg, and 200 mg/kg), M. exotica showed the activity of decreasing the contents of TNF-alpha and IL-1 beta in SF and the chondrocyte apoptosis in a dose-dependent manner. To investigate the probable mechanism, quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting were used to determine gene expression and protein profiles, respectively. The results reveal that M. exotica can downregulate mRNA and protein expressions of beta-catenin and COX-2 and reporter activity significantly. Conclusively, M. exotica exhibits antiapoptotic chondroprotective activity probably through inhibiting beta-catenin signaling.

• 出版日期2013
• 单位赣南医学院