RAPID, HIGH-LEVEL EXPRESSION OF BIOLOGICALLY-ACTIVE ALPHA-TRICHOSANTHIN IN TRANSFECTED PLANTS BY AN RNA VIRAL VECTOR

作者：KUMAGAI MH^*; TURPEN TH; WEINZETTL N; DELLACIOPPA G; TURPEN AM; DONSON J; HILF ME; GRANTHAM GL; DAWSON WO; CHOW TP; PIATAK M; GRILL LK

来源：Proceedings of the National Academy of Sciences of the United States of America, 1993, 90(2): 427-430.

DOI：10.1073/pnas.90.2.427

• SUBGENOMIC PROMOTER
• TOBAMOVIRUS
• RIBOSOME-INACTIVATING PROTEIN
• AIDS

Alpha-trichosanthin, a eukaryotic ribosome-inactivating protein from Trichosanthes kirilowii, inhibits the replication of the human immunodeficiency virus (HIV) in vitro. The alpha-trichosanthin gene was placed under the transcriptional control of a tobamovirus subgenomic promoter in a plant RNA viral vector. Two weeks after inoculation, transfected Nicotiana benthamiana plants accumulated alpha-trichosanthin to levels of at least 2% of total soluble protein. The recombinant alpha-trichosanthin was purified and its structural and biological properties were analyzed. The 23-amino acid signal peptide was recognized by N. benthamiana and the processed enzyme caused a concentration-dependent inhibition of protein synthesis in vitro. The high level of heterologous gene expression observed in these studies is due to the unique features of the RNA viral-based transfection system.

• 出版日期1993-1-15