A glial origin for periventricular nodular heterotopia caused by impaired expression of Filamin-A

作者：Carabalona Aurelie; Beguin Shirley; Pallesi Pocachard Emilie; Buhler Emmanuelle; Pellegrino Christophe; Arnaud Karen; Hubert Philippe; Oualha Mehdi; Siffroi Jean Pierre; Khantane Sabrina; Coupry Isabelle; Goizet Cyril; Gelot Antoinette Bernabe; Represa Alfonso; Cardoso Carlos^*

来源：Human Molecular Genetics, 2012, 21(5): 1004-1017.

DOI：10.1093/hmg/ddr531

Periventricular nodular heterotopia ( PH) is a human brain malformation caused by defective neuronal migration that results in ectopic neuronal nodules lining the lateral ventricles beneath a normal appearing cortex. Most affected patients have seizures and their cognitive level varies from normal to severely impaired. Mutations in the Filamin-A ( or FLNA) gene are the main cause of PH, but the underlying pathological mechanism remains unknown. Although two FlnA knockout mouse strains have been generated, none of them showed the presence of ectopic nodules. To recapitulate the loss of FlnA function in the developing rat brain, we used an in uteroRNA interference-mediated knockdown approach and successfully reproduced a PH phenotype in rats comparable with that observed in human patients. In FlnA-knockdown rats, we report that PH results from a disruption of the polarized radial glial scaffold in the ventricular zone altering progression of neural progenitors through the cell cycle and impairing migration of neurons into the cortical plate. Similar alterations of radial glia are observed in human PH brains of a 35-week fetus and a 3-month-old child, harboring distinct FLNA mutations not previously reported. Finally, juvenile FlnA-knockdown rats are highly susceptible to seizures, confirming the reliability of this novel animalmodel of PH. Our findings suggest that the disorganization of radial glia is the leading cause of PH pathogenesis associated with FLNA mutations. Rattus norvegicus FlnA mRNA ( GenBank accession number FJ416060).

• 出版日期2012-3-1