A high-density genetic linkage map of a black spruce (Picea mariana) x red spruce (Picea rubens) interspecific hybrid

作者：Kang Bum Yong; Major John E; Rajora Om P^*

来源：Genome, 2011, 54(2): 128-143.

DOI：10.1139/G10-099

Genetic maps provide an important genomic resource of basic and applied significance. Spruce (Picea) has a very large genome size (between 0.85 x 10(10) and 2.4 x 10(10) bp; 8.5-24.0 pg/IC, a mean of 17.7 pg/1C). We have constructed a near-saturated genetic linkage map for an interspecific backcross (BC(1)) hybrid of black spruce (BS; Picea mariana (Mill.) B. S. P.) and red spruce (RS; Picea rubens Sarg.), using selectively amplified microsatellite polymorphic loci (SAMPL) markers. A total of 2284 SAMPL markers were resolved using 31 SAMPL-MseI selective nucleotide primer combinations. Of these, 1216 SAMPL markers showing Mendelian segregation were mapped, whereas 1068 (46.8%) SAMPL fragments showed segregation distortion at alpha = 0.05. Maternal, paternal, and consensus maps consistently coalesced into 12 linkage groups, corresponding to the haploid chromosome number (1n = 1x = 12) of 12 in the genus Picea. The maternal BS map consisted of 814 markers distributed over 12 linkage groups, covering 1670 cM, with a mean map distance of 2.1 cM between adjacent markers. The paternal BS x RS map consisted of 773 markers distributed over 12 linkage groups, covering 1563 cM, with a mean map distance of 2.0 cM between adjacent markers. The consensus interspecific hybrid BC1 map consisted of 1216 markers distributed over 12 linkage groups, covering 1865 cM (98% genome coverage), with a mean map distance of 1.5 cM between adjacent markers. The genetic map reported here provides an important genomic resource in Picea, Pinaceae, and conifers.

• 出版日期2011-2