A method for estimating the glomerular filtration rate in conscious monkeys

作者：Satoh Hiroshi^*; Nomiya Nana; Imai Daiki; Sato Shigeru; Sakurai Ken; Takasuna Kiyoshi; Furuhama Kazuhisa

来源：Journal of Applied Toxicology, 2016, 36(2): 266-270.

DOI：10.1002/jat.3178

• cynomolgus monkey
• glomerular filtration rate
• iodixanol
• serum clearance

To establish a method for estimating the glomerular filtration rate (GFR) in conscious monkeys, the radiographic contrast medium iodixanol and the standard agent inulin were coadministered as tracers to male cynomolgus monkeys (Macaca fascicularis) as a bolus injection; blood was collected after 60, 90 and 120 min. An equation based on a single-blood-sample method derived from Jacobsson's formula was prepared using the data from healthy and saline-and gentamicin-treated monkeys by a multisample strategy with iodixanol. The GFR using the equation with iodixanol was in agreement with that from the multisamplemethod with inulin or iodixanol. When the GFR decreased to more than 60% of the basal reference level, serum creatinine concentrations tended to increase, whereas serum blood urea nitrogen concentrations fluctuated. The results suggest that the single-sample-blood method with iodixanol is a practical tool for estimating the monkey GFR in a toxicological research setting therefore minimizing animal sufferings.

• 出版日期2016-2