Mitochondrial DNA Deficiency in Ovarian Cancer Cells and Cancer Stem Cell-like Properties

作者：Huang, Ruixia; Wang, Junbai; Zhong, Yali; Liu, Yishan; Stokke, Trond; Trope, Claes G.; Nesland, Jahn M.; Suo, Zhenhe^*

来源：Anticancer Research, 2015, 35(7): 3743-3753.

• Mitochondrion
• mitochondrial DNA deficiency/dysfunction
• transcriptome sequencing/RNA deep-sequencing
• cancer stem cells
• CSCs
• cancer cell proliferation
• anti-apoptosis
• stemness
• western blotting
• RT-PCR
• immunocytochemistry

Background: A low quantity of mitochondrial DNA (mtDNA) is a risk factor in a variety of tumor types. However, it is unclear how mtDNA reduction influences tumor behavior. Material and Methods: mtDNA-deficient ovarian cancer cells were established by ethidium bromide (EtBr) treatment with additive combination of pyruvate and uridine. Results: The mtDNA-deficient cells had a low growth and colony-forming efficiency compared to the control cells. RNA sequencing revealed down-regulation of mitochondrion-related genes and up-regulation of genes related to cell proliferation and antiapoptosis. The expression of genes involved in cancer metastasis, proliferation, angiogenesis, drug resistance and cancer cell stemness were also up-regulated. Intriguingly, cancer stem cell markers CD90 and CD117 were both up-regulated by EtBr dose-dependently in both cell lines. Conclusion: MtDNA deficiency may induce ovarian cancer stem cell-like properties through different ways in vitro, therefore contributing to different tumor behaviors.

• 出版日期2015-7
• 单位郑州大学