A novel human-like collagen hemostatic sponge with uniform morphology, good biodegradability and biocompatibility

作者：Jiang, Xijuan; Wang, Ya; Fan, Daidi; Zhu, Chenhui; Liu, Lin; Duan, Zhiguang

来源：Journal of Biomaterials Applications, 2017, 31(8): 1099-1107.

DOI：10.1177/0885328216687663

• Human-like collagen
• glutamine transaminase
• freeze-drying
• uniform
• biodegradable
• biocompatibility
• hemostatic sponge

Biodegradable sponges, as a promising hemostatic biomaterial, has been clinically required over the past decades. Current hemostatic sponges are generally prepared by crosslinking and freeze-drying, but the quality control or biocompatibility is often unsatisfactory due to the freezing-caused morphological non-uniformity and the toxicity of raw materials or cross-linkers. The crosslinking often greatly retards the degradation of the sponges and thus affects the healing of the wound. In this work, we prepared a novel hemostatic sponge using human-like collagen and glutamine transaminase (non-toxic cross-linker) and optimized its morphology via "two-step'' freezing instead of conventional "one-step'' freezing. The resulting sponge showed a good biocompatibility in cytotoxicity and implantation tests and had a significant hemostatic effect in ear artery and liver injury models. Moreover, the sponge could be degraded high efficiently by several common enzymes in organisms (e.g. I collagenase, trypsase, and lysozyme), which means that the sponge can be easily digested by metabolism and can facilitate seamless healing. Finally, both the front and back of the sponge prepared via two-step freezing was more uniform in morphology than that prepared via one-step freezing. More importantly, two-step freezing can be used as a universal approach for preparation of diverse uniform biomaterials.

• 出版日期2017-3
• 单位西北大学