Association of Vitamin E with Rapid Thawing on Goat Semen

作者：Penitente Filho Jurandy Mauro; Oliveira Fabricio Albani; Jimenez Carolina Rodriguez; Carrascal Erly; Oliveira Dias Julio Cesar; Oliveira Gisele Dias; Silveira Renata Gomes; Silveira Camila Oliveira; Alves Torres Ciro Alexandre

来源：The Scientific World Journal, 2014, 2014: 964172.

DOI：10.1155/2014/964172

The aim of this study was to evaluate the effects of vitamin E associated with rapid thawing on cryopreserved goat semen. Two bucks were used and eight ejaculates per animal were collected using artificial vagina. Semen was diluted with the following treatments: BIOXCELL (control), BIOXCELL + Equex (sodium lauryl sulphate) and BIOXCELL + vitamin E 100 mu M. Semen was packaged into 0.25 mL straws and cooled at 5 degrees C for 1 hour. Freezing was performed in liquid nitrogen vapor (-155 degrees C) during 15 minutes. Then, the straws were immersed in liquid nitrogen (-196 degrees C). Straws were thawed at 38 C/60 seconds or at 60 C/7 seconds with immediate sperm analysis. Hypoosmotic swelling test was performed adding a 20 mu L aliquot of thawed semen to 1 mL of hypoosmotic solution (100 mOsm.Kg(-1)) followed by incubation during 60 minutes in water bath (38 degrees C). Vitamin E did not affect any studied parameters (P > 0.05). Nevertheless, defrosting rate of 60 degrees C/7 seconds improved sperm membrane functional integrity (P < 0.05). Current knowledge about goat semen cryopreservation is not sufficient to ensure high post-thawing recovery rates; thus, this study brings important data about using antioxidants and different thawing rates on cryopreservation process.

• 出版日期2014