Active immunization against macrophage migration inhibitory factor using a novel DNA vaccine prevents ovariectomy-induced bone loss in mice

作者：Onodera Shin^*; Oshima Shigeki; Nishihira Jun; Yasuda Kazunori; Tohyama Harukazu; Irie Kazuharu; Koyama Yoshikazu

来源：Vaccine, 2008, 26(6): 829-836.

DOI：10.1016/j.vaccine.2007.11.066

• macrophage migration
• inhibitory factor
• ovariectomy
• vaccine

Previous studies have demonstrated that mice deficient in the macrophage migration inhibitory factor (MIF) gene are protected from ovariectomy (OVX)-induced bone loss. We developed a novel MIF-deoxyribonucleic acid (DNA) vaccine by introducing oligonucleotides encoding a helper T epitope into the cDNA sequence of murine MIF Mice given the MIF-DNA vaccine produced high titers of autoantibody against MIF, and were protected from OVX-induced bone loss. Our results further support the hypothesis that MIF is involved in the pathomechanism of OVX-induced bone loss, and also show that active immunization against MIF using a DNA vaccine may be useful for the prophylactic treatment of postmenopausal osteoporosis.

• 出版日期2008-2-6