Development of a combined technique using a rapid one-step immunochromatographic assay and indirect competitive ELISA for the rapid detection of baicalin

作者：Paudel Madan Kumar; Putalun Wara****; Sritularak Boonchoo; Morinaga Osamu; Shoyama Yukihiro; Tanaka Hiroyuki^*; Morimoto Satoshi

来源：Analytica Chimica Acta, 2011, 701(2): 189-193.

DOI：10.1016/j.aca.2011.05.054

• Baicalin
• Scutellaria Radix
• Immunochromatographic strip
• Anti-baicalin monoclonal antibody
• Enzyme-linked immunosorbent assay

A colloidal gold conjugated anti-baicalin monoclonal antibody (anti-BA MAb) was prepared and used in an immunochromatographic assay (ICA) for BA in Scutellariae Radix and Kampo medicines. This competitive ICA uses an anti-BA MAb which shows a high specificity for BA and baicalein. Its advantages include a short assay time (15 min), no dependence on any instrumental systems, and it can detect BA in plant materials and Kampo medicines. The limit of detection for the ICA was found to be around 0.6 mu g mL(-1) of baicalin. Moreover, the usefulness of the combination of indirect competitive ELISA and the ICA using anti-BA MAb as a quality control method was confirmed for analysis of BA in Scutellariae Radix and Kampo medicines with a sufficient sensitivity (200 ng mL(-1) to 2 mu g mL(-1)), obtainable in an easy and timely manner.

• 出版日期2011-9-9