Proteomic profiling and identification in peritoneal fluid of children treated by peritoneal dialysis

作者：Raaijmakers Renske^*; Pluk Wendy; Schroder Cornelis H; Gloerich Jolein; Cornelissen Elisabeth A M; Wessels Hans J C T; Willems Johannes L; Monnens Leo A H; van den Heuvel Lambert P W J

来源：Nephrology Dialysis Transplantation, 2008, 23(7): 2402-2405.

DOI：10.1093/ndt/gfn212

• mass spectrometry
• peritoneal dialysis
• proteomics
• paediatric

Background. Proteomic technologies offer a high-throughput analysis of the expression of proteins in biological samples. The global analysis of the proteins in peritoneal dialysis (PD) fluid will provide a better understanding of the biological processes of the peritoneal membrane.
Methods. The dialysate of nine paediatric PD patients was collected from peritoneal equilibrium tests with 3.86% glucose. Proteins were separated on a 10% SDS-PAGE gel and in-gel digested with trypsin. Peptide mixtures were analysed using nanoLC-MS/MS and results were searched against the NCBI database.
Results. A total number of 189 proteins were identified in the PD fluid of nine patients, with 88 proteins shared by all patients. These 88 proteins accounted for 47% of the identified proteins and > 90% of the total protein content in the analysed samples. Proteins were subdivided into eight different classes according to function.
Conclusions. This study gives a representative overview of the proteins present in PD fluid. The proteins in PD fluid reflect plasma proteins as well as local peritoneal processes. Potentially interesting proteins are revealed.

• 出版日期2008-7