Analysis of the In Vivo Turnover of CD4+T-Cell Subsets in Chronically SIV-Infected Sooty Mangabeys

作者：Ortiz Alexandra M; Carnathan Diane G; Yu Joana; Sheehan Katherine M; Kim Peter; Reynaldi Arnold; Vanderford Thomas H; Klatt Nichole R; Brenchley Jason M; Davenport Miles P; Silvestri Guido^*

来源：PLos One, 2016, 11(5): e0156352.

DOI：10.1371/journal.pone.0156352

Aberrant turnover of memory CD4+ T-cells is central to Acquired Immunodeficiency Syndrome (AIDS) progression. Understanding the relationship between the turnover of CD4+ subsets and immunological homeostasis during simian immunodeficiency virus (SIV) infection in natural hosts may provide insight into mechanisms of immune regulation that may serve as models for therapeutic intervention in Human Immunodeficiency Virus (HIV)infected persons. Sooty mangabeys (SMs) have naturally evolved with SIV to avoid AIDS progression while maintaining healthy peripheral CD4+ T-cell counts and thus represent a model by which therapeutic interventions for AIDS progression might be elucidated. To assess the relationship between the turnover of CD4+ subsets and immunological homeostasis during SIV infection in non-progressive hosts, we treated 6 SIV-uninfected and 9 SIVinfected SMs with 2'-bromo-5'-deoxyuridine (BrdU) for 14 days and longitudinally assessed CD4+ T-cell subset turnover by polychromatic flow cytometry. We observed that, in SIVinfected SMs, turnover of CD4+ T-cell naive and central, transitional, and effector memory subsets is comparable to that in uninfected animals. Comparable turnover of CD4+ T-cell subsets irrespective of SIV-infection status likely contributes to the lack of aberrant immune activation and disease progression observed after infection in non-progressive hosts.

• 出版日期2016-5-26
• 单位NIH