Unraveling the molecular mechanism governing the tissue specific expression of IFN lambda R1

作者：Akhtar Hashaam^*; Hamming Ole Jensen; Jana Syed Umer; Akhtar Samar; Terczyn'ska Dyla Ewa; Siupka Piotr; Shafique Adeena; Hartmann Rune; Sadia Hajra

来源：Pakistan Journal of Pharmaceutical Sciences, 2016, 29(3): 795-799.

• IFNLR1
• signal peptide
• interferon lambda
• expression
• transcription factor binding sites (TBS)

The functional receptor for type III interferons (IFNs) is a heterodimer of IFNLR1 and IL10R2. IFNLR1 is expressed in a highly tissue specific manner, with epithelial and liver tissue as the prime expressing tissues in humans. However, knowledge about the molecular pathways responsible for regulating the expression of IFNLR1 is yet unknown. In this study, various bioinformatics tools were used to predict the scores of signal peptides of IFN lambda R1 and IFN alpha R1, which was considered as an important difference in the expression of both receptors or participation in regulating the IFNLR1 gene. In silico study revealed that the signal peptide of IFNaR1 had more potential than the signal peptide of IFN lambda R1 but changing the signal peptide of wild type IFN lambda R1 with the signal peptide of IFNaR1 in wet lab had barely shown any differences. Selective expression of IFN lambda R1 was considered to be a plus point towards the targeted anti-viral activity of IFN lambda s but artificial control on its expression will surely make IFN lambda s a better drug with enhanced activity. The results of this study may help us in contributing some understanding towards the mechanisms involved in the selective expression of IFNLR1 and exceptionalities involved.

• 出版日期2016-5