An Efficient Binary Solvent Mixture for Monoacylglycerol Synthesis by Enzymatic Glycerolysis

作者：Zhong, Nanjing; Li, Lin; Xu, Xuebing; Cheong, Lingzhi; Li, Bing^*; Hu, Songqing; Zhao, Xihong

来源：Journal of the American Oil Chemists' Society, 2009, 86(8): 783-789.

DOI：10.1007/s11746-009-1402-7

• Glycerolysis
• Monoacylglycerol
• Lipozyme TL IM
• Binary solvent medium
• Soybean oil

The present study was aimed at selecting an efficient binary solvent mixture for monoacylglycerol (MAG) synthesis by enzymatic glycerolysis of soybean oil. Solvent combinations of tert-butanol/isopropanol (v/v) at different ratios were studied. Of the investigated cases, tert-butanol:isopropanol at ratio 80:20 was the most suitable organic medium. The optimum conditions for MAG synthesis under the selected mixture were: water 10 wt% based on glycerol, Lipozyme TL IM 15 wt% based on oil and glycerol, weight ratio of solvent to oil 4:1, and molar ratio of glycerol to oil 3.5:1. Under these conditions with a 4-h reaction, the yield of MAG was 72.0% where the triacylglycerol (TAG) content was reduced to only 1.0% (based on acylglycerols). Fatty acid ester (FAE) formation from the solvents was very low in the final product (1.3% based on reaction mixture). The selected binary solvent mixture has good physical properties with low melting point (-26.5 A degrees C), which can avoid the risk of crystallization in practical operations.

• 出版日期2009-8
• 单位华南理工大学