Nobiletin-Ameliorated Lipopolysaccharide-Induced Inflammation in Acute Lung Injury by Suppression of NF-κB Pathway In Vivo and Vitro

作者：Li, Weifeng; Zhao, Ruoqi; Wang, Xiumei; Liu, Fang; Zhao, Jinmeng; Yao, Qing; Zhi, Wenbing; He, Zehong; Niu, Xiaofeng^*

来源：Inflammation, 2018, 41(3): 996-1007.

DOI：10.1007/s10753-018-0753-3

• nobiletin
• acute lung injury
• LPS
• NF-kappa B
• inflammation

Nobiletin (NOB), a citrus polymethoxy flavonoid, has been reported to exhibit anti-inflammatory, anti-cancer, and anti-insulin resistance activities. Although the anti-inflammatory activity of NOB already reported, its involvement in lung protection has not been reported. Thus, this study aimed to investigate the anti-inflammatory response of NOB in lipopolysaccharide (LPS)-stimulated A549 cells and LPS-induced acute lung injury (ALI) in mice. The animals were pre-treated with NOB (5, 10, and 20 mg/kg) or DEX (5 mg/kg) at 12 and 1 h before intranasal instillation of LPS. The severity of pulmonary injury was evaluated 6 h after LPS administration. Results suggested that treatment with NOB dramatically attenuated lung histopathological changes, wet-to-dry (W/D) ratio, myeloperoxidase (MPO) activity, the numbers of inflammatory cells, and TNF-alpha, IL-6, and NO in BALF induced by LPS. Furthermore, NOB also significantly inhibited the expression of iNOS and the phosphorylation of NF-kappa Bp65 and I kappa B alpha. In vitro, NOB inhibited NF-kappa B activation and TNF-alpha, IL-6 production in LPS-stimulated A549 cells. Taken together, these results indicated that NOB exhibited a protective effect on ALI, and the possible mechanism is involved in inhibiting NF-kappa B activation, subsequently inhibiting LPS-induced inflammatory response.

• 出版日期2018-6
• 单位南昌大学; 西安交通大学