Application of PacBio Single Molecule Real-Time (SMRT) sequencing in bacterial source tracking analysis during milk powder production

作者：Wang Jicheng; Zheng Yi; Xi Xiaoxia; Hou Qiangchuan; Xu Haiyan; Zhao Jie; Li Jing; Bian Yanfei; Ma Huimin; Wang Yanjie; Kwok Lai Yu; Zhang Heping; Sun Zhihong^*

来源：Food Control, 2018, 93: 226-234.

DOI：10.1016/j.foodcont.2018.05.044

• Single molecule real-time (SMRT)
• Milk powder
• Bacterial source tracking
• In-house production
• Large-scale factory production

This work developed a 16S rRNA-PacBio Single Molecule Real-Time (SMRT) sequencing-based method to identify and track the bacterial community of milk powder (MP) from two kinds of production settings, i.e., small-scale production contained within an in-house environment (minimal milk storage before pasteurization, milk concentration, and spray drying) and a large-scale factory production (prolonged milk storage before direct spray drying). A total of 18 samples were analyzed at the species level. Comparing with the large-scale factory production, only relatively little changes were observed in the bacterial community during the in-house production process, without significant loss in the levels of bioactive minor proteins (namely, lactoferrin, immunoglobulin G, lactoperoxidase, and lysozyme). The two most prevalent species in the in-house production, Bacillus cereus and Bacillus flexus, were likely originated from the raw milk with only small changes in their relative abundances (from 25.97% to 26.40%-28.89% and 27.40%, respectively) throughout the processing (from raw milk to MP). In contrast, large-scale factory production resulted in more obvious variation in the microbial content. This microbial tracking approach is valuable in identifying the contamination source and the specific stage when contamination happens; the implementation of such technique may also enhance food quality assurance systems that are currently used in the dairy industry.

• 出版日期2018-11
• 单位内蒙古农业大学