Modulation of mitochondrial respiratory capacity by carrier-mediated transfer of RNA in vivo

作者：Jash Sukanta; Chowdhury Tapas; Adhya Samit^*

来源：Mitochondrion, 2012, 12(2): 262-270.

DOI：10.1016/j.mito.2011.10.001

• Mitochondria
• RNA
• Gene therapy
• In vivo
• Muscle respiration
• Aging

Genetic dysfunction of mitochondria is pathological, but an effective method of nucleic acid delivery to mitochondria in vivo is lacking. Injection into rodents of tagged polycistronic RNAs (pcRNAs) encoding parts of the organelle genome and bound to a carrier complex, resulted in rapid uptake and concentration of the RNA in many tissues. The delivered RNA was localized to mitochondria. A pan-genomic cocktail of pcRNAs restored mRNA levels, stimulated mitochondrial translation and respiratory capacity of skeletal muscle of middle-aged and old rats. Thus, the carrier-based protocol may be suitable for delivery of functional RNAs to mitochondria in vivo.

• 出版日期2012-3