A novel single-cell DNA fingerprinting method successfully distinguishes sibling human embryos

作者：Treff Nathan R^*; Su Jing; Tao Xin; Miller Kathleen A; Levy Brynn; Scott Richard T Jr

来源：Fertility and Sterility, 2010, 94(2): 477-484.

DOI：10.1016/j.fertnstert.2009.03.067

• Blastomere
• DNA fingerprinting
• DNA microarray
• single nucleotide polymorphism
• whole genome amplification

Objective: To validate a novel system for embryonic DNA fingerprinting which can reliably distinguish sibling embryos from each other.
Design: Prospective, randomized, and blinded study.
Setting: Academic center for reproductive medicine.
Patient(s): Blastomeres were obtained from discarded and transferred embryos from six patients undergoing IVF treatment.
Intervention(s): None.
Main Outcome Measure(s): Single lymphocytes from sibling cell lines and blastomeres from sibling day 3 human embryos were evaluated for accurate assignment of relationship using whole genome amplification and single-nucleotide polymorphism microarrays.
Result(s): Assignment of single-cell relationships was accomplished with 100% accuracy. We also observed complete agreement between the molecular karyotype and DNA fingerprint-based identification of embryos implanted in three clinical IVF cases after multiple embryo transfer.
Conclusion(s): These data demonstrate the first single-blastomere DNA fingerprinting technology capable of unequivocal discrimination of sibling human embryos. (Fertil Steril(R) 2010;94:477-84.

• 出版日期2010-7