Detection of an Antibody to Avian Influenza Virus by an Electrochemical Immunoassay (eELISA)

作者:Ohtsuka Keiichi; Endo Hiroshi; Morimoto Kimio; Vuong Bui N; Ogawa Haruko; Imai Kunitoshi; Takenaka Shigeori*
来源:Analytical Sciences, 2008, 24(12): 1619-1622.
DOI:10.2116/analsci.24.1619

摘要

The detection of an antibody against nucleoprotein (NP) of the avian influenza virus (AIV) was attempted by an electrochemical immunoassay by combining a secondary antibody-alkaline phosphatase conjugate and 4-aminophenylphosphate. Released 4-aminophenol was quantitated electrochemically, and the current derived from oxidation of the hydrolysis product increased linearly over a wide primary antibody concentration range (10 - 1000 ng/ml). The detection limit of this electrochemical immunoassay for the antibody against the NP of AIV was found to be 10 ng/ml.

  • 出版日期2008-12