An AhR-Luciferase Adenovirus Infection System for Rapid Screening of Dioxins in Soils

作者：Shy Cherng Gueih; Chao How Ran^*; Lin Ding Yan; Gou Yan You; Chuang Chun Yu; Chuang Kuo Pin; Chen Chih Cheng; Hsiao Yung Chun; Chen Fu An^*

来源：Bulletin of Environmental Contamination and Toxicology, 2016, 96(2): 192-196.

DOI：10.1007/s00128-015-1684-4

• Ad-DR bioassay
• Dioxins
• Aryl hydrocarbon receptor (AhR)
• Luciferase
• Soil

Our goal was to develop a fast-screening method for measuring dioxin levels in soils. The adenovirus (Ad)-dioxin-responsive (DR) bioassay system (AdEasy-6XDRE-TATA-Luc) combined with a fast-cleanup system was examined under conventional conditions (i.e., with incubation at 37A degrees C) and three alternative conditions [incubation at 37A degrees C with addition of phorbol-12-myristate-13-acetate (PMA), incubation at 33A degrees C, and incubation at 33A degrees C with addition of PMA]. The best conditions for carrying out the Ad-DR bioassay was 33A degrees C and no addition of PMA. The background level of soil dioxins determined by the chemical assay [6.49 ng I-TEQ/kg dry weight (dw)] was well correlated (Pearson's r = 0.873, p < 0.001) with that by the Ad-DR bioassay [expressed in ng bioanalytical equivalents (BEQ) 81.1 ng BEQ/kg dw] (n = 17). When surveyed in contaminated soil samples (n = 114) from industrial areas by the Ad-DR bioassay, dioxin levels were 117, 102, 98.5, and 112 ng BEQ/kg dw, respectively, in northern, central, southern, and eastern Taiwan.

• 出版日期2016-2
• 单位长春大学; 清华大学