Adding Gas Chromatography-Mass Spectrometry Data to a Melting Point and Thin-Layer Chromatography Laboratory

作者：Azman Adam M; Barrett Julie A; Darragh Megan; Esteb John J; McNulty LuAnne M; Morgan Paul M; O'Reilly Stacy A; Wilson Anne M^*

来源：Journal of Chemical Education, 2013, 90(1): 140-141.

DOI：10.1021/ed300490x

• Second-Year Undergraduate
• Laboratory Instruction
• Organic Chemistry
• Hands-On Learning/Manipulatives
• Problem Solving/Decision Making
• Gas Chromatography
• Mass Spectrometry
• Physical Properties
• Thin Layer Chromatography

The addition of gas chromatography-mass spectrometry (GC-MS) data interpretation to a thin-layer chromatography (TLC) and melting point (mp) laboratory for an introductory organic course is described. Students are given a sample containing one unknown and a list of 24 unknown compounds, organized in groups by similar mp ranges. Students identify the unknown through mp and TLC. Students are then given GC-MS data containing three unknowns and are asked to determine GC peak corresponding to their unknown using the MS spectra. Upon completion of the laboratory, students have gained an understanding of how many components are present given GC-MS data and how to identify each component from the molecular ion. They also become familiar with the physical characteristics of melting point, retention factor, and retention time. These skills are utilized in other experiments throughout the academic year

• 出版日期2013-1