Epitope screening of the PCV2 Cap protein by use of a random peptide-displayed library and polyclonal antibody

作者：Ge, Meng; Yan, Ai; Luo, Wei; Hu, Yun-Fei; Li, Run-Cheng; Jiang, Da-Liang; Yu, Xing-Long^*

来源：Virus Research, 2013, 177(1): 103-107.

DOI：10.1016/j.virusres.2013.06.018

• Porcine circovirus 2
• Capsid protein
• Phage display
• Epitope mapping
• Polyclonal antibodies

Capsid protein (Cap), the only structural protein of porcine circovirus 2 (PCV2), is involved in the host protective response and is a target for vaccine development. To find a rapid and easy way to fully map the antigenic epitopes of Cap, purified Cap-specific polyclonal antibodies were used to screen a random heptapeptide phage display library. After three rounds of screening, twenty phage clones that had binding activity to Cap-specific antibodies (tested by phage ELISA) were sequenced. When the inserted amino acid sequences were aligned with the Cap protein sequence, eight core regions in Cap ((50)SRTFGYT(56), (VRTPSW67)-V-62, (68)AVDMMR(73), (79)FLPPGG(84), (SNPRSVPF93)-S-86, (KVEFWP107)-K-102, (119)GSSXXXLDDN(128) and (PPLNP233)-P-229) were identified, three of which ((50)SRTFGYT(56), (SNPRSVPF93)-S-86 and (KVEFWP107)-K-102) for the first time. Nine phagetopes representing the eight regions were chosen to immunize Kunming mice. All except minotopes (50)SRTFGYT(56) and (PPLNP233)-P-229 induced antibodies against PCV2 when injected into Kunming mice.

• 出版日期2013-10
• 单位湖南农业大学