Use of adenovirus vector expressing the mouse full estrogen receptor alpha gene to infect mouse primary neurons

作者：Hu, Xiao; Lou, Lei; Yuan, Jun; Wan, Xing; Wang, Jianyi; Qin, Xinyue^*

来源：Neural Regeneration Research, 2010, 5(15): 1138-1143.

DOI：10.3969/j.issn.1673-5374.2010.15.003

• estrogen receptor alpha
• adenovirus
• neural cell
• C57BL/6 mice
• gene transfection
• nerve factor
• neural regeneration

Estrogen plays important regulatory and protective roles in the central nervous system through estrogen receptor a mediation. Previous studies applied eukaryotic expression and lentiviral vectors carrying estrogen receptor a to clarify the underlying mechanisms. In the present study, an adenovirus vector expressing the mouse full estrogen receptor a gene was constructed to identify biological characteristics of estrogen receptor a recombinant adenovirus infecting nerve cells. Primary cultured mouse nerve cells were first infected with estrogen receptor a recombinant adenovirus at various multiplicities of infection, followed by 100 multiplicity of infection. Results showed overexpression of estrogen receptor a mRNA and protein in the infected nerve cells. Estrogen receptor a recombinant adenovirus at 100 multiplicity of infection successfully infected neurons and upregulated estrogen receptor a mRNA and protein expression.

• 出版日期2010-8-15
• 单位重庆医科大学; 遵义医科大学; 贵州省人民医院