MicroRNA-127-3p promotes glioblastoma cell migration and invasion by targeting the tumor-suppressor gene SEPT7

作者：Jiang, Huawei; Hua, Dasong; Zhang, Jing; Lan, Qing; Huang, Qiang; Yoon, Jae-Geun; Han, Xu; Li, Lisha; Foltz, Gregory; Zheng, Shu^*; Lin, Biaoyang

来源：Oncology Reports, 2014, 31(5): 2261-2269.

DOI：10.3892/or.2014.3055

• miR-127-3p
• SEPT7
• glioblastoma
• migration

MicroRNAs (miRNAs) are small non-coding RNAs of 20-25 nucleotides in length that are capable of modulating gene expression post-transcriptionally. The potential roles of miRNAs in the tumorigenesis of glioblastoma (GBM) have been under intensive studies in the past few years. In the present study, we found a positive correlation between the levels of miR-127-3p and the cell migration and invasion abilities in several human GBM cell lines. We showed that miR-127-3p promoted cell migration and invasion of GBM cells using in vitro cell lines and in vivo mouse models. We identified SEPT7, a known tumor-suppressor gene that has been reported to suppress GBM cell migration and invasion, as a direct target of miR-127-3p. SEPT7 was able to partially abrogate the effect of miR-127-3p on cell migration and invasion. In addition, microarray analysis revealed that miR-127-3p regulated a number of migration and invasion-related genes. Finally, we verified that miR-127-3p affected the remodeling of the actin cytoskeleton mediated by SEPT7 in GBM cells.

• 出版日期2014-5
• 单位苏州大学; 浙江大学