The bc:caa(3) supercomplexes from the Gram positive bacterium Bacillus subtilis respiratory chain: A megacomplex organization?

作者：Sousa Pedro M F; Videira Marco A M; Santos Filipe A S; Hood Brian L; Conrads Thomas P; Melo Ana M P^*

来源：Archives of Biochemistry and Biophysics, 2013, 537(1): 153-160.

DOI：10.1016/j.abb.2013.07.012

• Bacillus subtilis
• Respiratory chain supercomplex
• bc:caa(3) Megacomplex
• Nitrate reductase
• Oxidative phosphorylation

The respiratory chain of some prokaryotes was shown to be organized in supercomplexes. This association has been proposed to improve enzyme stability and the overall efficiency of the oxidative phosphorylation process. Here, we have revisited recent data on the supercomplexes of Bacillus subtilis respiratory chain, by means of 1D and 2D-BN-PAGE, sucrose gradient fractionation of solubilized membranes, and mass spectrometry analysis of BN-PAGE bands detected in gel for succinate and cytochrome c oxidoreductase activities. The cytochrome bc:caa(3) oxygen oxidoreductase supercomplex was observed in different stoichiometries, (bc)(4):(caa(3))(2), (bc)(2):(caa(3))(4) and 2[(bc)(2):(caa(3))(4)], suggesting for the first time the string association model of supercomplexes in a Gram positive bacterium. In addition, the presence of a succinate:quinone oxidoreductase:nitrate reductase supercomplex was confirmed by the co-localized succinate:nitroblue tetrazolium and methylviologen:nitrate oxidoreductase activities detected in gel and corroborated by LC-MS/MS analysis.

• 出版日期2013-9-1