Use of a stable-isotope-labeled reporter peptide and antioxidants for reliable quantification of methionine oxidation in amonoclonal antibody by liquid chromatography/mass spectrometry

作者：Kang Pilsoo; Mezhebovsky Tanya; Chen Wei; McCardle James A; Zhang Sheng; Sullivan Eric P; Sass Philip M; Routhier Eric^*

来源：Rapid Communications in Mass Spectrometry, 2016, 30(14): 1734-1742.

DOI：10.1002/rcm.7646

RATIONALE: Accurate quantification of methionine oxidation in therapeutic proteins by liquid chromatography/mass spectrometry (LC/MS) is challenging due to the potential artifacts introduced during sample preparation and analysis in the peptide mapping workflow. In this study, a systematic approach for optimization of the peptide mapping procedure to achieve reliable quantification of endogenous methionine oxidation in monoclonal antibodies was developed.
METHODS: The approach is based on usage of a stable-isotope-labeled reporter peptide, identical in sequence to the tryptic peptide of an IgG1 monoclonal antibody containing the methionine residue most prone to oxidation. This approach was applied to evaluating various desalting procedures, and tested on nanoLC/MS, microLC/MS and UPLC/MS for the peptide mapping analysis of a model monoclonal antibody IgG1 sensitive to oxidation.
RESULTS: Several steps in the peptide mapping procedure with LC/MS detection at which protein oxidation occurred were identified and optimized using the reference stable-isotope-labeled peptide. Thus, reliable quantification of methionine oxidation in the target monoclonal antibody was validated.
CONCLUSIONS: The methodology which utilizes the reference stable-isotope-labeled reporter peptide is applicable to monoclonal antibody oxidation analysis and could be extended to other biotherapeutics once oxidation-prone methionine(s) in the protein sequence are identified.

• 出版日期2016-7-30