Nematicidal Activities of 4-Quinolone Alkaloids Isolated from the Aerial Part of Triumfetta grandidens against Meloidogyne incognita

作者：Jang Ja Yeong; Quang Le Dang; Choi Yong Ho; Choi Gyung Ja; Jang Kyoung Soo; Cha Byeongjin; Ngoc Hoang Luu; Kim Jin Cheol^*

来源：Journal of Agricultural and Food Chemistry, 2015, 63(1): 68-74.

DOI：10.1021/jf504572h

• Meloidogyne incognita
• nematicidal activity
• Triumfetta grandidens
• 4-quinolone alkaloids

The methanol extract of the aerial part of Triumfetta grandidens (Tiliaceae) was highly active against Meloidogyne incognita, with second-stage juveniles (J2s) mortality of 100% at 500 mu g/mL at 48 h post-exposure. Two 4-quinolone alkaloids, waltherione E (1), a new alkaloid, and waltherione A (2), were isolated and identified as nematicidal compounds through bioassay-guided fractionation and instrumental analysis. The nematicidal activities of the isolated compounds against M. incognita were evaluated on the basis of mortality and effect on egg hatching. Compounds 1 and 2 exhibited high mortalities against J2s of M. incognita, with EC50 values of 0.09 and 0.27 mu g/mL at 48 h, respectively. Compounds 1 and 2 also exhibited a considerable inhibitory effect on egg hatching, which inhibited 91.9 and 87.4% of egg hatching, respectively, after 7 days of exposure at a concentration of 1.25 mu g/mL. The biological activities of the two 4-quinolone alkaloids were comparable to those of abamectin. In addition, pot experiments using the crude extract of the aerial part of T. grandidens showed that it completely suppressed the formation of gall on roots of plants at a concentration of 1000 mu g/mL. These results suggest that T. grandidens and its bioactive 4-quinolone alkaloids can be used as a potent botanical nematicide in organic agriculture.

• 出版日期2015-1-14