Direct observation of CD4 T cell morphologies and their cross-sectional traction force derivation on quartz nanopillar substrates using focused ion beam technique

作者：Kim Dong Joo; Kim Gil Sung; Hyung Jung Hwan; Lee Won Yong; Hong Chang Hee; Lee Sang Kwon^*

来源：Nanoscale Research Letters, 2013, 8(1): 332.

DOI：10.1186/1556-276X-8-332

• Cell traction force
• Cell adhesion
• CD4 T cell
• Cell migration
• Focused ion beam

Direct observations of the primary mouse CD4 T cell morphologies, e.g., cell adhesion and cell spreading by culturing CD4 T cells in a short period of incubation (e.g., 20 min) on streptavidin-functionalized quartz nanopillar arrays (QNPA) using a high-content scanning electron microscopy method were reported. Furthermore, we first demonstrated cross-sectional cell traction force distribution of surface-bound CD4 T cells on QNPA substrates by culturing the cells on top of the QNPA and further analysis in deflection of underlying QNPA via focused ion beam-assisted technique.

• 出版日期2013-7-23