科研之友
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摘要
A cost-efficient frequency domain system for measuring nanosecond fluorescence lifetime has been designed and tested. In this design, function generators are adopted to directly modulate a light-emitting diode at radio frequencies. A heterodyne detection technique is used to reduce the bandwidth of the system and to improve the signal-to-noise ratio. A fluorescence lifetime close to 2 ns was measured and verified by comparison with the results from a broadband lock-in amplifier. The total cost of the system is reasonable for undergraduate laboratories, which enables students to gain hands-on experience in designing and constructing a nanosecond fluorescence lifetime measurement system. Some suggestions for conducting the measurement with a limited budget are provided.
- 出版日期2010-1
