Algal-bacterial process for the simultaneous detoxification of thiocyanate-containing wastewater and maximized lipid production under photoautotrophic/photoheterotrophic conditions

作者：Ryu Byung Gon; Kim Jungmin; Farooq Wasif; Han Jong In; Yang Ji Won; Kim Woong^*

来源：Bioresource Technology, 2014, 162: 70-79.

DOI：10.1016/j.biortech.2014.03.084

• Thiocyanate wastewater
• Algal-bacterial mixed culture
• Lipid productivity
• Photoautotrophic/photoheterotrophic conditions
• Volatile fatty acid

In this work, a cooperative algal-bacterial system that efficiently degrades thiocyanate (SCN-), a toxic contaminant, and exhibits high lipid productivity, was developed. A consortium of mixed bacteria (activated sludge) and microalgae was sequentially cultivated under photoautotrophic and photoheterotrophic modes. The hydrolysis of SCN- to ammonium (NH4+)-nitrogen and subsequent nitrification steps were performed by the initial activated sludge under lithoautotrophic conditions. The NH4+ and oxidized forms of nitrogen, nitrite (NO2-) and nitrate (NO3-), were then assimilated and removed by the microalgal cells when light was supplied. After the degradation of SCN-, the cultivation mode was changed to photoheterotrophic conditions in a sequential manner. Algal-bacterial cultures containing Chlorella protothecoides and Ettlia sp. yielded significantly increased lipid productivity under photoheterotrophic conditions compared to photoautotrophic conditions (28.7- and 17.3-fold higher, respectively). Statistical methodologies were also used to investigate the effects of volatile fatty acids and yeast extract on biomass and lipid production.

• 出版日期2014-6