Attenuation of lung cancer stem cell tumorigenesis and metastasis by cisplatin

作者：Wang, Shenxu; Ma, Sai; Li, Xiujuan; Xue, Zengfu; Zhang, Xiaotian; Fan, Weiwei; Nie, Yongzhan; Wu, Kaichun; Chen, Xiaoyuan; Cao, Feng^*

来源：Experimental Lung Research, 2014, 40(8): 404-414.

DOI：10.3109/01902148.2014.938201

• cancer stem cells
• cisplatin
• growth factor
• lung cancer
• molecular imaging

Purpose: To investigate the effect of cisplatin on the growth and metastasis abilities of lung cancer stem cells (CSCs) via molecular imaging. Materials and Methods: The expression changes of lung CSCs cellmarker in A549-Luc- C8 human non-small-cell lung cancer (NSCLC) cell line with or without cisplatin treatment were detected by flow cytometry. The tumorigenesis and metastasis abilities of A549-Luc-C8 cells were monitored both in vitro and in vivo, and the mechanism was assessed by gene sequencing. Results: About 1%-2% of CSCs were detected in A549-Luc-C8 cells and decreased CSCs percentage was observed after cisplatin treatment. Attenuated tumorigenesis and metastasis abilities of A549-Luc-C8 cells were found in cisplatin treated group. Conclusions: Decreased percentage of CSCs in A549-Luc-C8 cells can be induced by cisplatin treatment, which may partly be attributed to the attenuated expression of growth factors.

• 出版日期2014-10
• 单位中国人民解放军总医院; 中国人民解放军空军军医大学; 中国人民解放军第150中心医院; 第四军医大学; 厦门大学