Development of a test kit for β-lactam antibiotic residues in milk

作者：Liu, Dong-Mei; Guo, Jun; Liang, Jie-Ying; Wu, Hui; Yu, Yi-Gang; Li, Xiao-Feng; Tang, Yu-Qian

来源：Modern Food Science and Technology, 2014, 30(10): 251-256.

DOI：10.13982/j.mfst.1673-9078.2014.10.042

• Antibiotic residues
• Bacillus stearothermophilus
• Bromocresol purples
• Culture temperature
• Growth conditions
• Milk
• Optimum reaction temperature
• Single-factor experiments

We used β-lactam antibiotic residues in milk as the target to develop a rapid detection kit. Based on sensitivity of Bacillus stearothermophilus 10208 to acid and β-lactam antibiotics, growth conditions were optimized and a test kit that could show a color reaction within 2.5 h was developed. The results of the single-factor experiment showed that when the inoculum was 5%, initial growth pH was 8.0, culture temperature was 55, and the quantity of peptone was 0.50%, the logarithm values of growth for the bacterium were 2.9, 7.5, 7.3, and 5.8, respectively. The results of an orthogonal test showed that when a nutrient broth containing 0.50% peptone was added and the mixture was inoculated with 5% seed broth and incubated for 24 h at 65, the logarithm value of growth for the bacterium was 7.6. This optimized test kit could be used to detect residual β-lactam antibiotics in milk within 2.5 h. The parameters were as follows: reaction volume, 0.5 mL;agar, 1.5%;bromocresol purple, 0.01 g/L;total BS 10208 cell number, 1.30 ×108cfu, and optimum reaction temperature, 65. The detection limit on penicillin sodium was 2~4 ¶g/L. The test kit could also detect residual tetracyclines, sulfonamides, macrolides, aminoglycosides, and other antibiotics in milk.

• 出版日期2014-10-15
• 单位华南理工大学