Adjuvanted HLA-supertype restricted subdominant peptides induce new T-cell immunity during untreated HIV-1-infection

作者：Karlsson Ingrid; Brandt Lea; Vinner Lasse; Kromann Ingrid; Andreasen Lars Vibe; Andersen Peter; Gerstoft Jan; Kronborg Gitte; Fomsgaard Anders^*

来源：Clinical Immunology, 2013, 146(2): 120-130.

DOI：10.1016/j.clim.2012.12.005

• CAF01 adjuvant
• HIV-1
• HLA-supertypes
• Subdominant epitopes
• Therapeutic vaccine

We investigated the potential of inducing additional T-cell immunity during chronic HIV-1 infection directed to subdominant HIV-1 epitopes from common HLA-supertypes. Ten treatment-naive HIV-1-infected individuals were immunized with peptides in the adjuvant CAF01. One individual received placebo. T-cell immunogenicity was examined longitudinally by a flow cytometry (CD107a, IFN gamma, TNF alpha, IL-2 and/or MIP1 beta expression) as well as IFN gamma ELISPOT. Safety was evaluated by clinical follow up combined with monitoring of biochemistry, hematology, CD4 T-cell counts and viral load. New CD4 and CD8 T-cell responses specific for one or more vaccine epitopes were induced in 10/10 vaccinees. The responses were dominated by CD107a and MIP1 beta expression. There were no significant changes in HIV-1 viral load or CD4 T-cell counts. Our study demonstrates that the peptide/CAF01 vaccine is safe and that it is possible to generate new HIV-1 T-cell responses to defined epitopes in treatment-naive HIV-1-infected individuals.

• 出版日期2013-2