Methicillin-resistant Staphylococcus epidermidis carrying biofilm formation genes: detection of clinical isolates by multiplex PCR

作者：Iorio Natalia L P; Azevedo Milena B; Frazao Vanessa H; Barcellos Ariane G; Barros Elaine M; Pereira Eliezer M; de Mottos Claudio S; dos Santos Katia R N^*

来源：International Microbiology, 2011, 14(1): 13-17.

DOI：10.2436/20.1501.01.130

• Staphylococcus epidermidis
• molecular identification
• methicillin resistance gene
• biofilm formation genes
• multiplex PCR

Staphylococcus epidermidis is the most prevalent coagulase-negative Staphylococcus (CNS) and is a major cause of hospital bacteremia. Based on 18 reference strains and 149 Staphylococcus clinical strains, used in a novel multiplex PCR method, the aim of this study was to identify S. epidermidis with respect to the sequence of three genes: recN, which encodes a recombination/repair protein, mecA (methicillin resistance), and icaAB, which is involved in biofilm formation. Amplicons of 219 bp (S. epidermidis-recN gene), 154 bp (mecA gene), and 546 bp (icaAB genes) were obtained. Reliable results were achieved for 100% of the evaluated strains, suggesting that this new multiplex-PCR approach could be useful for the accurate identification of methicillin-resistant S. epidermidis with the potential to produce biofilm. [Int Microbiol 2011; 14(1):13-17]

• 出版日期2011-3