Morphologic and immunohistochemical characterization of granulomas in the nucleotide oligomerization domain 2-related disorders Blau syndrome and Crohn disease

作者：Janssen Carl E I; Rose Carlos D; De Hertogh Gert; Martin Tammy M; Meunier Brigitte Bader; Cimaz Rolando; Harjacek Miroslav; Quartier Pierre; Ten Cate Rebecca; Thomee Caroline; Desmet Valeer J; Fischer Alain; Roskams Tania; Wouters Carine H^*

来源：Journal of Allergy and Clinical Immunology, 2012, 129(4): 1076-1084.

DOI：10.1016/j.jaci.2012.02.004

• Nucleotide oligomerization domain 2
• Blau syndrome
• Crohn disease
• granuloma
• T(H)17 cell
• monocyte macrophage lineage
• multinucleated giant cell

Background: Blau syndrome (BS) and Crohn disease (CD) are both characterized by granulomatous inflammation and related to nucleotide oligomerization domain 2 (NOD2) mutations. %26lt;br%26gt;Objective: This study aimed to define the morphologic and immunohistochemical characteristics of granulomas in patients with NOD2-related BS and CD. %26lt;br%26gt;Methods: Granuloma-containing biopsy specimens from 6 patients with BS and 7 pediatric patients with CD carrying NOD2 mutations or single nucleotide polymorphisms were studied for morphology, cellular composition, and cytokine expression by using hematoxylin and eosin staining and immunohistochemistry. %26lt;br%26gt;Results: Biopsy specimens from patients with BS typically showed polycyclic granulomas with large lymphocytic coronas, extensive emperipolesis of lymphocytes within multinucleated giant cells (MGCs), MGC death, and fibrinoid necrosis and fibrosis. In contrast, biopsy specimens from patients with CD showed simple granulomas with subtle/absent lymphocytic coronas, sclerosis of the surrounding tissue, and polymorphonuclear cells. Findings found to be similar in all granulomas were as follows: CD68 and HLA-DR expression by epithelioid cells, monocyte-macrophage lineage cells and MGCs, increased lymphocytic HLA-DR expression, increased CD4(+)/CD8(+) T-cell ratio, and CD20(+) B lymphocytes evenly distributed within and around granulomas. In both patient groups prominent IFN-gamma expression was found in and around granulomas, and TNF-alpha and IL-23 receptor expression was moderate. IL-6, IL-17, and TGF-beta expression was prominent in granulomas from patients with BS but sporadic in granulomas from patients with CD. IL-10 expression was absent. %26lt;br%26gt;Conclusion: Granulomas from patients with BS and granulomas from patients with NOD2-associated CD show distinct morphologic features and cytokine expression patterns, suggesting that the T(H)17 axis might be involved in the pathogenesis of BS, whereas T(H)1 is important in both patients with BS and patients with CD. (J Allergy Clin Immunol 2012;129:1076-84.)

• 出版日期2012-4