Amino Acid Sequences of Two Immune-Dominant Epitopes of Recoverin Are Involved in Ca2+/Recoverin-Dependent Inhibition of Phosphorylation of Rhodopsin

作者：Senin I I^*; Tikhomirova N K; Churumova V A; Grigoriev I I; Kolpakova T A; Zinchenko D V; Philippov P P; Zernii E Yu

来源：Biochemistry-Moscow, 2011, 76(3): 332-338.

DOI：10.1134/S0006297911030060

• Ca2+-dependent antibodies
• Ca2+-myristoyl switch
• cancer associated retinopathy
• recoverin

Antibodies AB(60-72) and AB(80-92) against two immune-dominant epitopes of photoreceptor Ca2+-binding protein recoverin, 60-DPKAYAQHVFRSF-72 and 80-LDFKEYVIALHMT-92, which can be exposed in a Ca2+-dependent manner, were obtained. The presence of AB(60-72) or AB(80-92) results in a slight increase in Ca2+-affinity of recoverin and does not affect significantly a Ca2+-myristoyl switch mechanism of the protein. However in the presence of AB(60-72) or AB(80-92) recoverin loses its ability to interact with rhodopsin kinase and consequently to perform a function of Ca2+-sensitive inhibitor of rhodopsin phosphorylation in photoreceptor cells.

• 出版日期2011-3