A Reverse Genetics Approach To Study Feline Infectious Peritonitis

作者：Tekes Gergely; Spies Danica; Bank Wolf Barbara; Thiel Volker^*; Thiel Heinz Juergen

来源：Journal of Virology, 2012, 86(12): 6994-6998.

DOI：10.1128/JVI.00023-12

Feline infectious peritonitis (FIP) is a lethal immunopathological disease caused by feline coronaviruses (FCoVs). Here, we describe a reverse genetics approach to study FIP by assessing the pathogenicity of recombinant type I and type II and chimeric type I/type II FCoVs. All recombinant FCoVs established productive infection in cats, and recombinant type II FCoV (strain 79-1146) induced FIP. Virus sequence analyses from FIP-diseased cats revealed that the 3c gene stop codon of strain 79-1146 has changed to restore a full-length open reading frame (ORF).

• 出版日期2012-6