Sixteen novel microsatellite loci developed for the giant panda (Ailuropoda melanoleuca)

作者：Zhang He Min; Guo Yang; Li De Sheng; Wang Peng Yan; Fang Sheng Guo^*

来源：Conservation Genetics, 2009, 10(3): 589-592.

DOI：10.1007/s10592-008-9576-3

• Genetic diversity
• Allele
• Polymorphism
• Exclusion probability

Sixteen novel microsatellite DNA loci were developed from the giant panda (Ailuropoda melanoleuca) using a magnetic-bead capture method. A total of 115 alleles were obtained for these markers, ranging from 4 to 12 alleles per locus (average 7.188). These loci exhibited high levels of polymorphic information content and expected heterozygosity, 0.558-0.855 (average 0.729) and 0.628-0.885 (average 0.778), respectively. Therefore, the allelic polymorphism and heterozygosity show that the giant pandas raised in China Research and Conservation Center possess abundant genetic variation. In addition, if the three markers showing null alleles were excluded, the remaining 13 microsatellite loci still presented extremely low non-exclusion probabilities of parentage (0.002), paternity (0.000) and identity (0.000). As a result, this new suit of microsatellite markers would be a very informative tool for the genetic and conservation studies of giant pandas.

• 出版日期2009-6
• 单位浙江大学; 中国林业科学研究院大熊猫研究中心