Size effect of Se-enriched green tea particles on in vitro antioxidant and antitumor activities

作者：Li Huajia; Li Feng; Yang Fangmei; Fang Yong; Xin Zhihong; Zhao Liyan; Hu Qiuhui^*

来源：Journal of Agricultural and Food Chemistry, 2008, 56(12): 4529-4533.

DOI：10.1021/jf0731200

• superfine Se-enriched green tea
• particle size
• antioxidant
• antitumor

The antioxidant and antitumor activities (in vitro) of superfine regular and Se-enriched green tea particles with different sizes (3.52 mu m and 220 nm) were investigated in this paper. The vitamin C and tea polyphenol contents of green tea in different sizes were significantly different, and amino acid and chlorophyll just changed a little. The antioxidant activity of green tea particles was evaluated by DPPH radical scavenging and linoleic acid peroxidation inhibition methods, and the antitumor activity was evaluated by anti proliferation assay on HepG2, A549, and MGC803 cells. The results indicated that enrichment of selenium endowed green tea with higher antioxidant activity and antitumor activity on HepG2 and A549 cells but not on MGC803 cells. The DPPH radical scavenging rates of regular and Se-enriched green tea of 220 nm (67.87% and 69.49%, respectively) were significantly greater than that of 3.52 mu m, but the inhibition of linoleic acid peroxidation for green tea of 220 nm was lower. The inhibitory rates of green tea of 220 nm on HepG2, A549, and MGC803 cells achieved 77.35%, 80.76%, and 87.54% for regular green tea, and 82.51%, 88.09%, and 74.48% for Se-enriched green tea at the dose of 100 mu g mL(-1), values that were all significantly higher compared to that of 3.52 mu m.

• 出版日期2008-6-25
• 单位南京农业大学