Measurement of excited singlet oxygen molecule in a vacuum sterilization system, using electric spin resonance (ESR) with a water-soluble polymer thin film

作者：Yoshino Kiyoshi^*; Iwamori Satoru

来源：Journal of Photochemistry and Photobiology A: Chemistry , 2016, 328: 148-153.

DOI：10.1016/j.jphotochem.2016.04.009

• Active oxygen species
• Sterilization
• Excited singlet oxygen molecule
• Polyvinyl alcohol
• Electron spin resonance
• Ultraviolet irradiation

An advanced sterilization system employing active oxygen species (AOS) was investigated. We revealed in a previous paper that, by using active oxygen species generated from oxygen gas by ultraviolet irradiation in the chamber of the sterilization system, the survival curves of Geobacillus stearothermophilus spores (10(6) CFU), a biological indicator (BI), showed exponential reduction, and that this sterilization was attributable to the UV-generated AOS. However, we had little knowledge of what specific types of AOS were primarily responsible for the sterilization effects. Therefore, in this study we investigated the principal sterilization contributors among the AOS generated by ultraviolet irradiation, using the electron spin resonance (ESR) technique, with 2,2,6,6-tetramethyl-4-piperidinol (TEMP) as a spin-label reagent for the excited state of atomic oxygen molecules (O-1(2)), and compared the respective sterilization effects of AOS and oxygen plasma system.

• 出版日期2016-9-1