Limited Susceptibility of Cynomolgus Monkeys (Macaca fascicularis) to Leprosy after Experimental Administration of Mycobacterium leprae

作者：Walsh Gerald P; Dela Cruz Eduardo C; Abalos Rodolfo M; Tan Esterlina V; Fajardo Tranquilino T; Villahermosa Laarni G; Cellona Roland V; Balagon Maria V; White Valerie A; Saunderson Paul R; Walsh Douglas S^*

来源：The American Journal of Tropical Medicine and Hygiene, 2012, 87(2): 327-336.

DOI：10.4269/ajtmh.2012.11-0780

Cynomolgus monkeys are a useful model for human tuberculosis, but susceptibility to M. leprae is unknown. A cynomolgus model of leprosy could increase understanding of pathogenesis importantly, neuritis and nerve-damaging reactions. We administered viable Mycobacterium leprae to 24 cynomolgus monkeys by three routes, with a median follow-up period of 6 years (range = 1-19 years) involving biopsies, nasal smears, antiphenolic glycolipid-1 (PGL-1) antibody serology, and lepromin skin testing. Most developed evanescent papules at intradermal M. leprae inoculation sites that, on biopsy, showed a robust cellular immune response akin to a lepromin skin test reaction; many produced PGL-1 antibodies. At necropsy, four monkeys, without cutaneous or gross neurological signs of leprosy but with elevated PGL-1 antibodies, including three with nasal smears (+) for acid fast bacilli (AFB), showed histological features, including AFB, suggestive of leprosy at several sites. Overall, however, cynomolgus monkeys seem minimally susceptible to leprosy after experimental M. leprae administration.

• 出版日期2012-8
• 单位河北医科大学