Decoding regulatory landscape of somatic embryogenesis reveals differential regulatory networks between japonica and indica rice subspecies

作者：Indoliya Yuvraj; Tiwari Poonam; Chauhan Abhisekh Singh; Goel Ridhi; Shri Manju; Bag Sumit Kumar; Chakrabarty Debasis

来源：Scientific Reports, 2016, 6(1): 23050.

DOI：10.1038/srep23050

<jats:title>Abstract</jats:title><jats:p>Somatic embryogenesis is a unique process in plants and has considerable interest for biotechnological application. Compare to <jats:italic>japonica</jats:italic>, <jats:italic>indica</jats:italic> rice has been less responsive to <jats:italic>in vitro</jats:italic> culture. We used Illumina Hiseq 2000 sequencing platform for comparative transcriptome analysis between two rice subspecies at six different developmental stages combined with a tag-based digital gene expression profiling. Global gene expression among different samples showed greater complexity in <jats:italic>japonica</jats:italic> rice compared to <jats:italic>indica</jats:italic> which may be due to polyphyletic origin of two rice subspecies. Expression pattern in initial stage indicate major differences in proembryogenic callus induction phase that may serve as key regulator to observe differences between both subspecies. Our data suggests that phytohormone signaling pathways consist of elaborate networks with frequent crosstalk, thereby allowing plants to regulate somatic embryogenesis pathway. However, this crosstalk varies between the two rice subspecies. Down regulation of positive regulators of meristem development (i.e. <jats:italic>KNOX</jats:italic>, <jats:italic>OsARF5</jats:italic>) and up regulation of its counterparts (<jats:italic>OsRRs</jats:italic>, <jats:italic>MYB</jats:italic>, <jats:italic>GA20ox1</jats:italic>/<jats:italic>GA3ox2</jats:italic>) in <jats:italic>japonica</jats:italic> may be responsible for its better regeneration and differentiation of somatic embryos. Comprehensive gene expression information in the present experiment may also facilitate to understand the monocot specific meristem regulation for dedifferentiation of somatic cell to embryogenic cells.</jats:p>

• 出版日期2016-3-14