Multidrug-resistant epidemic clones among bloodstream isolates of Pseudomonas aeruginosa in the Czech Republic

作者：Nemec Alexandr^*; Krizova Lenka; Maixnerova Martina; Musilek Martin

来源：Research in Microbiology, 2010, 161(3): 234-242.

DOI：10.1016/j.resmic.2010.02.002

• Pseudomonas aougazosa
• Bloodstream infection
• Population structure
• Interhospital transmission
• MLST
• Integrons
• Macrorestriction analysis.
• Serotyping

To determine whether the high proportion of antimicrobial resistance among hospital isolates of Pseudomonas aerugmosa in the Czech Republic is associated with the spread of multidrug-resistant clones, we investigated 108 bloodstream Isolates collected prospectively in 2007 The isolates originated from 48 hospitals in 36 cities and were serotyped, tested for susceptibility to 10 anti-Pseudomonas agents and studied by multdocus sequence typing. macrorestnction analysis and class I inteciron typing Forty-five isolates were fully susceptible, while 14 and 49 Isolates were resistant to 1-2 and 3-9 agents, respectively A total of 42 mululocus sequence types (ST) were identified, of which ST235 (serotype 011). STI75 (04) and STI32 (06) Included 19, 16 and 5 isolates, respectively These three STs encompassed 40(82%) of 49 isolates resistant to more than two agents and originated from 29 hospitals in 22 cities Isolates of the same ST had highly similar macrorestriction patterns Twelve ST235 Isolates harbored an mtegron variable region with the gene cassette array of aacA7-aadA6-orfD, while 15 STI75 isolates shared a region with the aadB-aadA13 array and all STI32 Isolates carried a region with aacA4. A carbapenemase-encoding gene (b/aimp_7) was detected in a single strain (ST357) In conclusion, the multidrug resistance of Czech P aer

• 出版日期2010-4